The majority of RNA-seq library preparation protocols quantify the expression of genes without taking into account the numerous transcript variants which contribute to this overall expression. SQUARE mRNA-seq uses a selective matrix to separate transcript variants and to enable the assembly and quantification of individual transcripts. The protocol is highly specific for full-length, polyadenylated mRNA, and transcript hypotheses are supported by directional transcription start and polyadenylation site tagging.
SPLIT kit delivers high quality total RNA or small and large RNA fractions for sensitive Next Generation Sequencing.
Today Lexogen has launched the SPLIT RNA Extraction Kit which allows extraction of either 24 small (< 150 nt) and 24 large (> 150 nt) RNA fractions or 48 fractions of total RNA. The protocol does not require DNase treatment. The isolated RNA sample has very high quality, particularly RIN score of 8.0-9.5, no genomic DNA contamination, and no degraded RNA due to DNase treatment. Thus the SPLIT RNA Extraction Kit is highly recommended for using in Next Generation Sequencing where quality of RNA sample is essential, as well as for other high demanding applications such as full-length reverse transcription or sample preparation for microarray analysis.
Ultra strand-specific, fast and reproducible mRNA-Seq library prep is now available for Illumina, Ion Torrent and SOLiD sequencing
Lexogen is glad to announce that users of Ion Torrent and SOLiD can now also benefit from the SENSE mRNA-Seq Library Prep Kit. The SENSE mRNA-Seq library prep has exceptional strand-specificity (>99.9%) and therefore enables excellent detection and accurate quantification of antisense transcripts and overlapping genes. The very short and simple workflow allows producing ready-to-sequence libraries from low amounts of total RNA within just 4 hours. Because SENSE does not require fragmentation and has just few steps, potential biases usually present in multistep protocols are eliminated, thus providing high reproducibility of the protocol.