Over a century ago, Louis Pasteur invented the glass Pasteur pipette to reduce contamination when transferring samples. The Pasteur pipette is still in use today. The next significant improvement on pipettes occurred in the late 1950s with the introduction of a handheld, piston-operated pipette as a safe alternative to potentially dangerous mouth pipetting. The first handheld pipettes had pre-established volume setting (fixed volume pipettes). Further improvement was provided with the more flexible stepper volume setting (variable volume pipettes). In 1972, Dr. Warren Gilson invented the first continuously adjustable pipette. As an original error-preventing feature, the selected volume was now clearly displayed on the Gilson pipette (direct digital readout). Today, Gilson precision pipettes are still the world standard for accuracy, precision, and reliability.Today, the company still maintains its continuous effort of creativity and offers innovative, robust, and reliable pipettes to help scientists in their daily work.The pipetting system is our core expertise, and we truly enjoy sharing this knowledge and experience with pipette users so that they may achieve their goals.This guide will provide you with keys to understand how pipettes should be used and to get the most out of them. Examples given are based on our own pipette range, but the techniques described are equally applicable to other brands of pipettes and tips.