QuantSeq 3‘mRNA-Seq Library Prep Kit for Illumina [PDF]
QuantSeq 3‘mRNA-Seq Library Prep Kit generates Illumina-compatible libraries of the sequences close to the 3’end of the polyadenylated RNA. Only one fragment per transcript is generated and therefore no length normalization of reads is needed. This results in extremely accurate gene expression values. QuantSeq is available in two versions: using the first kit (Cat. No. 015.96) NGS reads are generated towards the oligodT, and to pinpoint the exact 3’ end paired-end sequencing may be required. This version of QuantSeq results in reads directly reflecting the mRNA sequence. With the second version of the kit (Cat.No. 016.96) it is possible to exactly pinpoint the 3’ end during Read 1. The reads generated here during Read 1 reflect the cDNA sequence. In order to achieve cluster calling on the Illumina platform a T-fill reaction is required first for this version of the kit. QuantSeq maintains exceptional strand-specificity of more than 99.9 % and allows to map reads to their corresponding strand on the genome, enabling the discovery and quantification of antisense transcripts and overlapping genes.