QuantSeq 3'mRNA-Seq kit
The new QuantSeq 3’mRNA-Seq Library Prep is the best alternative for microarrays and conventional RNA-Seq for gene expression analysis and eQTL studies, and a great tool for detection of the 3'UTR. Lexogen has launched the QuantSeq 3’mRNA-Seq Library Prep kit. This protocol generates Illumina-compatible libraries of the 3' end of transcripts from 100 pg - 2 µg of total RNA within 4.5 hours. As only one fragment per transcript is produced no length normalization is required, and an accurate determination of gene expression values is possible. Sequencing from the poly(A) tail makes it furthermore possible to assess the exact 3'UTR. By maintaining the strand-specificity (> 99.9%) the protocol allows an efficient detection of the antisense transcripts, overlapping and fusion genes. With the high level of multiplexing (96 samples/lane) and the low price per reaction, QuantSeq is an cost-efficient alternative for gene expression analysis.