VTU Technology is a leading contract research and development company located in Austria, providing services for the fast track generation of high performance, industrial protein production strains and economically viable protein production processes. The company offers time-saving development and optimization of cost-efficient recombinant Pichia pastoris protein production processes for biopharmaceutical products and enzymes. The development activities include production strain generation, development of upstream and downstream processes as well as establishment of analytical procedures and production of protein samples.
VTU has established a broad range of technologies and know-how for the generation of high performance Pichia protein production strains and processes. Exclusive proprietary technologies and profound experience of the VTU team lead to competitive production processes for a wide range of recombinant proteins.
An exclusive library of synthetic PAOX1 promoter variants – VTU´s highly approved 1st generation promoter variants - forms the core of VTU´s cutting-edge in-house Pichia pastoris toolbox enabling high-level protein production and secretion of more than 20 g/L for a variety of proteins by individual fine-tuning of expression. Notably, this library was complemented with groundbreaking and unique methanol-free 2nd generation PAOX1 promoter variants, facilitating strong expression even with just glycerol or glucose as the sole carbon source clearly outperforming conventional promoter systems not depending on methanol.
The versatility and effectiveness of VTU´s Pichia system is further underlined by a set of proprietary expression enhancing helper factors, several platform strains with different genetic backgrounds, elaborated cloning and transformation protocols, a high-throughput microscale screening and cultivation regime and effective fermentation protocols for maximization of product yield and overall process performance.
Judging from numerous completed and ongoing development projects, protein production processes developed in VTU´s labs have been shown to be robust and scalable facilitating sound technology transfer and straightforward implementation in large scale production facilities.
To date several VTU customers have licensed and successfully scaled-up such processes for the manufacture of recombinant proteins with VTU´s Pichia system to volumes ranging from several hundred to more than 10,000 L.