medorex e.K. 23. Januar 2014

Bioreactor for Small-Scale Investigations

A Novel Conical Shaped Bioreactor for Small-Scale Investigations under Defined Condition The reliability of metabolic or kinetic data determined in small-scale culture systems is often insufficient, as these systems cannot be run continuously and parameters such as dissolved oxygen and pH are not controlled. Here a new conical shaped bioreactor (medorex, Germany) for the cultivation of animal cells is presented. The reactor can be operated at a volume range of about 50 ml to a maximum volume of 400 ml. The conical shape allows the integration of probes and various tubes even at small volumes. The reactor has all features of a conventional glass reactor with up to 9 openings for inlet, outlet, level control and sampling, temperature sensor, an exhaust air cooler and ports for dissolved oxygen and pH probes. Low shear stress mixing is achieved by a 3- blade propeller stirrer with a diameter of 36 mm. Temperature can be controlled over a water jacket with a conventional water bath via the internal sensor. The main applications are: • studies of batch and fed-batch cultures at small scale • expanding cell cultures in fed-batch under defined conditions (pH, DO) • long-term continuous cultures at high dilution rates for kinetic studies with low medium consumption. The reactor can be modified to a fixed-bed reactor of 20 ml packed bed volume for the cultivation of adherent cell lines and testing of different carrier material.
medorex wurde 2004 als kleines innovatives Unternehmen in der Nähe von Göttingen in Südniedersachen gegründet. Wir sind ein hoch motivie... [mehr]