The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech offers high-performance PureCube NTA Agarose, for purification of his-tagged proteins. - Highest binding capacity on the market (up to 70 mg/mL) - Superior DTT and EDTA stability (up to 10 mM / 1.5 mM, respectively) - Can be regenerated for reuse - Available as Ni-NTA, Co-NTA or loaded with other transition metals - Matching anti-his antibody available PureCube Ni-NTA Agarose is produced with a highly cross-linked agarose which is physically very stable and suitable for purification processes under low pressure (bulk purifications as well as packed in chromatography columns). Additionally, the high porosity and uniform size of the matrix allows for optimal protein interaction and high reproducibility between individual purification runs.
The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. Cube Biotech offers PureCube Glutathione Agarose and prepacked, FPLC-compatible columns for efficient purification of GST-tagged proteins. For purification from cell culture supernatants or pull-down experiments, we recommend PureCube Glutathione MagBeads. - protein binding capacity of up to 10 mg/ mL - compatible with a range of detergents and additives - Matching GST antibody now available!
Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. - Membrane scaffold proteins (MSPs) of different lengths: Our MSPs are available with an N-terminal 6xHis tag, or without any tag. They are lyophilized in 2 mg aliquots. For other variants, please contact us. - Nanodisc assembly kits: Can be used for direct reconstitution of proteins in cell-free expression experiments. One kit contains 2 mg lyophilized MSP, and the needed amounts of phospholipid (e.g. DMPC) and sodium cholate, conveniently prealiquoted and with a detailed protocol. - Pre-assembled nanodiscs: Available in different compositions and quantities. - Nanodisc services: As part of our membrane protein services, proteins can be expressed directly into nanodiscs in cell-free reactions, or reconstituted after purification. Contact us to discuss your project.
Affinity chromatography is typically done with agarose beads with particle sizes of 40-150 µm. This material is very well suited for gravity flow and FPLC applications. However, for special applications, agaroses with exceptionally large bead sizes (300-500 µm in diameter) are more useful than the standard products. These include: - Automated purification using pipetting robots: Large beads sediment fast by gravity, and bead carryover and bead loss during pipetting are minimized - Purifying uncleared lysates: Residual particles do not clog the material. For quick screening, Agaroses XL can be used directly after cell lysis, without clearing the lysate by centrifugation. This procedure saves time and equipment, but results in slightly reduced purity. Features of our Agarose XL products are comparable to our standard agaroses, and the following modifications are available: - His affinity matrices (Ni-NTA, Ni-IDA, Co-NTA, and unloaded matrices) with binding capacities of 35-40 µg/ml agarose and resistance to DTT and EDTA - Glutathione affinity matrices - Strep-Tactin matrices - Rho1D4 matrices for membrane protein purifications - Activated matrices for coupling of biomolecules - Customized matrices All our products are manufactured in our own facilities, with all due care and high lot-to-lot reproducibility to ensure optimal results in your laboratory or manufacturing unit.
Special Affinity Agaroses For non-standard applications, Cube Biotech offers a broad range of modified affinity agaroses. Even these can be further modified depending on your individual needs. Similar modifications are available for magnetic beads. - Agarose-based affinity matrices from 20 to 500 µm particle sizes - Pre-activated matrices for coupling to proteins or other molecules - Highly reproducible synthesis in our own laboratories, or external sourcing of special materials - Off the shelf and tailor-made for your applications
Proteins tagged with the epitope sequence of the rho1D4 antibody can be purified with this affinity matrix. Cube Biotech produces the first commercially available immunoaffinity resin for the Rho1D4 purification system. - demonstrably suitable for membrane protein research - binding capacity of 3-4 mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse - matching antibody available For dilute samples or pull-down experiments, PureCube Rho1D4 MagBeads are available, featuring the same surface chemistry.